Journal: Biology of reproduction

Article Title: In utero and in vitro proteinase activity during the Mesocricetus auratus embryo zona escape time window.

doi: 10.1095/biolreprod64.1.222

Figure Lengend Snippet: FIG. 4. Zymograms containing a series of representative uterine flushates from pregnant (a) and pseudopregnant (b) hamsters. Uterine flushates were collected over the time period from 66 to 80 h PEA (see Materials and Methods). The enzyme activity in lanes P-UF 67, P-UF 68, and P-UF 69 PEA in the pregnant and Vx-UFa 73, Vx-UFb 73, and Vx-UFa 74 PEA in the pseudopregnant females are representative enzyme activity banding patterns from different animals when there are no correlative positive bioassays. The enzyme activity in lanes P-UF 70 and P-UF 71 PEA in pregnant and Vx-UFb 74, Vx-UFa 75, and Vx-UFb 75 PEA in pseudopregnant females are representative enzyme activity banding patterns from different animals when there are positive correlative bioassays (Fig. 1 and Table 1). For other enzyme banding patterns that were demonstrated by zymography with positive correlative bioassays, see text.

Article Snippet: Zymography Discontinuous dual slab polyacrylamide gels (Mini-Protean II Dual Slab Cell; BioRad, Hercules, CA), and buffers were prepared according to Laemmli [30].

Techniques: Activity Assay, Zymography